Biology of Hepatocellular Carcinoma are Restrained through PI3K/AKt Signaling Pathways and Targeted by Tumor Infiltrating Macrophages

To study the biology of hepatocellular carcinoma are restrained through PI3K/AKt signaling pathways and targeted by tumor infiltrating macrophages.Methods: Mononuclear cells were isolated from healthy adult peripheral blood by density gradient centrifugation and induced with IL 4 for selective activation of macrophagesin vitro.PI3K siRNA and AKT – siRNA were transfected to hepatocellular carcinoma cell line HepG2 respectively by lipofectermine 3000, and then cultivate with selective activation of macrophages (M2) for 48 hour. PI3K and Akt mRNA expression level in liver cancer HepG2 cells were detected with real-time fluorescent quantitative PCR. Results: After transfection, PI3K, and Akt mRNA level were significantly decreased. In vitro microenvironment of liver cancer, M2 can significantly increase levels of PI3K, and Akt mRNA levels in HepG2 cell lines, and can significantly promote the HepG2 cell proliferation ability (P< 0.05) after co-culture with M2 for 24 hours. Conclusion: Tumor associated macrophages can promote liver cancer cell proliferation through PI3K/Akt signaling pathway. If changing the microenvironment of liver cancer or specific inhibition of PI3K/Akt signaling pathway can also control the malignant biological behavior of tumors.